Search results for "Numerical aperture"
showing 10 items of 20 documents
Three-dimensional multiple-particle tracking with nanometric precision over tunable axial ranges
2017
The precise localization of nanometric objects in three dimensions is essential to identify functional diffusion mechanisms in complex systems at the cellular or molecular level. However, most optical methods can achieve high temporal resolution and high localization precision only in two dimensions or over a limited axial (z) range. Here we develop a novel wide-field detection system based on an electrically tunable lens that can track multiple individual nanoscale emitters in three dimensions over a tunable axial range with nanometric localization precision. The optical principle of the technique is based on the simultaneous acquisition of two images with an extended depth of field while …
Speckle random coding for 2D super resolving fluorescent microscopic imaging.
2006
In this manuscript we present a novel super resolving approach based upon projection of a random speckle pattern onto samples observed through a microscope. The projection of the speckle pattern is created by coherent illumination of the inspected pattern through a diffuser. Due to local interference of the coherent wave front with itself, a random speckle pattern is superimposed on the sample. This speckle pattern can be scanned over the object. A super-resolved image can be extracted from a temporal sequence of images by appropriate digital processing of the image stream. The resulting resolution is significantly higher than the diffraction limitation of the microscope objective. The new …
On the optimum form of an aperture for a confinement of the optically excited electric near field.
2008
Summary A triangular nanoaperture in an aluminium film was used previously as a probe in a scanning near-field optical microscope to image single fluorescent molecules with an optical resolution down to 30 nm. The high-resolution capability of the triangular aperture probe is because of a highly confined spot of the electric near field which emerges at an edge of the aperture, when the incident light is polarized perpendicular to this edge. Previous numerical calculations of the near-field distribution of a triangular aperture in a planar metal film using the field susceptibility technique yielded a nearly quantitative agreement with the experimental results. Using the same numerical techni…
Computational imaging with a balanced detector
2016
Single-pixel cameras allow to obtain images in a wide range of challenging scenarios, including broad regions of the electromagnetic spectrum and through scattering media. However, there still exist several drawbacks that single-pixel architectures must address, such as acquisition speed and imaging in the presence of ambient light. In this work we introduce balanced detection in combination with simultaneous complementary illumination in a single-pixel camera. This approach enables to acquire information even when the power of the parasite signal is higher than the signal itself. Furthermore, this novel detection scheme increases both the frame rate and the signal-to-noise ratio of the sys…
Three-ring filters increase the effective NA up to 1.46 in optical sectioning fluorescence microscopy
2003
Single-photon fluorescence confocal microscopy techniques can be combined with the use of specific binary filters in order to increase their optical sectioning capability. We present a novel class of axially super-resolving binary pupil filters specially designed to reach this aim. These filters let us to obtain a relevant compression of the z-response together with the reduction of the photo-bleaching effect typically inherent to apodization techniques. The fact of joining both the three-ring filters we propose in the illumination path, and the confocal detection gives rise to an important effective increase of lenses of effective numerical aperture.
Reduction of the spherical aberration effect in high-numerical-aperture optical scanning instruments.
2006
In modern high-numerical-aperture (NA) optical scanning instruments, such as scanning microscopes, optical data storage systems, or laser trapping technology, the beam emerging from the high-NA objective focuses deeply through an interface between two media of different refractive index. Such a refractive index mismatch introduces an important amount of spherical aberration, which increases dynamically when scanning at increasing depths. This effect strongly degrades the instrument performance. Although in the past few years many different techniques have been reported to reduce the spherical aberration effect, no optimum solution has been found. Here we concentrate on a technique whose mai…
Metalenses with high-NA, enhanced resolution and apodization
2017
Dielectric microlenses employed in imaging and focusing for optoelectronics are currently prospects to be substituted by metalenses showing an extraordinary optical performance within notably reduced volumes. Here we present some proposals to achieve an enhanced resolution in metallodielectric metasurface-based lenses established on either efficient arrangements with high numerical aperture or spatial filtering enabling to surpass the limit of resolution derived by the Rayleigh criterion.
Large Depth-of-Field Integral Microscopy by Use of a Liquid Lens
2018
Integral microscopy is a 3D imaging technique that permits the recording of spatial and angular information of microscopic samples. From this information it is possible to calculate a collection of orthographic views with full parallax and to refocus computationally, at will, through the 3D specimen. An important drawback of integral microscopy, especially when dealing with thick samples, is the limited depth of field (DOF) of the perspective views. This imposes a significant limitation on the depth range of computationally refocused images. To overcome this problem, we propose here a new method that is based on the insertion, at the pupil plane of the microscope objective, of an electrical…
A Model to Characterize the D-T Layer of ICF Targets by Backlit Optical Shadowgraphy
2005
A numerical model is presented in order to modelize the bright ring that appears in backlit optical shadowgraphy on a transparent hollow sphere with a solid deuterium-tritium layer inside. This novel model is based on computational calculations applied to the problem of the targets used in inertial confinement fusion. The model takes into account the influences of the optical imaging system (numerical aperture, source divergence, camera resolution, etc.) and the effect of the capsule itself, diameter, thickness, and refractive index, and allows one to analyze the inner surface of a capsule in terms of thickness and roughness.
Cryogenic setup for trapped ion quantum computing
2016
We report on the design of a cryogenic setup for trapped ion quantum computing containing a segmented surface electrode trap. The heat shield of our cryostat is designed to attenuate alternating magnetic field noise, resulting in 120~dB reduction of 50~Hz noise along the magnetic field axis. We combine this efficient magnetic shielding with high optical access required for single ion addressing as well as for efficient state detection by placing two lenses each with numerical aperture 0.23 inside the inner heat shield. The cryostat design incorporates vibration isolation to avoid decoherence of optical qubits due to the motion of the cryostat. We measure vibrations of the cryostat of less t…